CP009313.1

Detailed information

Microorganism
Streptomyces nodosus strain ATCC 14899

Taxonomy
Phylum : Actinobacteria Class : nan Order : Streptomycetales Family : Streptomycetaceae Genus : Streptomyces

Isolation Source
Orinoco river basin

Enzyme Name
Nitrate reductase

Protein Information
Pro_GenBank I.D： AJE38868.1 Length：543 aa Protein FASTA_sequence: Link

Information about Article
Reference：Sweeney et al., 2016 Title：Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production Pubmed ID：26497174.0 Pubmed link： Link Full research link： Link Abstract：The genome of the amphotericin producer Streptomyces nodosus was sequenced. A single scaffold of 7,714,110 bp was obtained. Biosynthetic genes were identified for several natural products including polyketides, peptides, siderophores and terpenes. The majority of these clusters specified known compounds. Most were silent or expressed at low levels and unlikely to compete with amphotericin production. Biosynthesis of a skyllamycin analogue was activated by introducing expression plasmids containing either a gene for a LuxR transcriptional regulator or genes for synthesis of the acyl moiety of the lipopeptide. In an attempt to boost amphotericin production, genes for acyl CoA carboxylases, a phosphopantetheinyl transferase and the AmphRIV transcriptional activator were overexpressed, and the effects on yields were investigated. This study provides the groundwork for metabolic engineering of S. nodosus strains to produce high yields of amphotericin analogues.

Reference：Sweeney et al., 2016
Title：Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production
Pubmed ID：26497174.0
Pubmed link： Link
Full research link： Link
Abstract：The genome of the amphotericin producer Streptomyces nodosus was sequenced. A single scaffold of 7,714,110 bp was obtained. Biosynthetic genes were identified for several natural products including polyketides, peptides, siderophores and terpenes. The majority of these clusters specified known compounds. Most were silent or expressed at low levels and unlikely to compete with amphotericin production. Biosynthesis of a skyllamycin analogue was activated by introducing expression plasmids containing either a gene for a LuxR transcriptional regulator or genes for synthesis of the acyl moiety of the lipopeptide. In an attempt to boost amphotericin production, genes for acyl CoA carboxylases, a phosphopantetheinyl transferase and the AmphRIV transcriptional activator were overexpressed, and the effects on yields were investigated. This study provides the groundwork for metabolic engineering of S. nodosus strains to produce high yields of amphotericin analogues.