AY425154.1

Detailed information

Microorganism
Uncultured bacterium

Taxonomy
Phylum : nan Class : nan Order : nan Family : nan Genus : nan

Isolation Source
Activated sludge

Enzyme Name
nitrate reductase

Protein Information
Pro_GenBank I.D： AAR18962.1 Length：142 aa Protein FASTA_sequence: Link

Information about Article
Reference：Throback et al., 2004 Title：Reassessing PCR Primers Targeting nirS, nirK and nosZ Genes for Community Surveys of Denitrifying Bacteria With DGGE Pubmed ID：19712290.0 Pubmed link： Link Full research link： Link Abstract：We re-evaluated PCR primers targeting nirS, nirK and nosZ genes for denaturing gradient gel electrophoresis as a tool to survey denitrifying community composition in environmental samples. New primers for both nirS and nosZ were combined with existing primers, while for nirK the previously published F1aCu:R3Cu set was chosen for denaturing electrophoresis. All three sets yielded amplicons smaller than 500 bp and amplified the correct fragment in all environmental samples. The denaturing gradient gel electrophoresis worked satisfactorily for nirK and nosZ, but not for nirS. This was probably due to the multiple melting domains in this particular nirS fragment. From the excised and sequenced bands, only sequences related to the target genes were detected and tree analysis showed that the selected primers acted as broad range primers for each of the three genes. By use of the new nirS primers it was demonstrated that agricultural soil harbours a substantial diversity of nirS denitrifiers.

Reference：Throback et al., 2004
Title：Reassessing PCR Primers Targeting nirS, nirK and nosZ Genes for Community Surveys of Denitrifying Bacteria With DGGE
Pubmed ID：19712290.0
Pubmed link： Link
Full research link： Link
Abstract：We re-evaluated PCR primers targeting nirS, nirK and nosZ genes for denaturing gradient gel electrophoresis as a tool to survey denitrifying community composition in environmental samples. New primers for both nirS and nosZ were combined with existing primers, while for nirK the previously published F1aCu:R3Cu set was chosen for denaturing electrophoresis. All three sets yielded amplicons smaller than 500 bp and amplified the correct fragment in all environmental samples. The denaturing gradient gel electrophoresis worked satisfactorily for nirK and nosZ, but not for nirS. This was probably due to the multiple melting domains in this particular nirS fragment. From the excised and sequenced bands, only sequences related to the target genes were detected and tree analysis showed that the selected primers acted as broad range primers for each of the three genes. By use of the new nirS primers it was demonstrated that agricultural soil harbours a substantial diversity of nirS denitrifiers.