Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Uncultured bacterium

Taxonomy

  • Phylum : nan
  • Class : nan
  • Order : nan
  • Family : nan
  • Genus : nan

Isolation Source

Coastal microbial mat

Enzyme Name

NirS

  • Encoding Gene:NirS
  • DNA Size:392 bp
  • Nucleotide FASTA sequence: Link

  • UniProt I.D: nan

Protein Information

  • Pro_GenBank I.D: AIG15425.1

  • Length:131 aa
  • Protein FASTA_sequence: Link

Information about Article

  • Reference:Fan et al., 2015
  • Title:Denitrification and the denitrifier community in coastal microbial mats.
  • Pubmed ID:25764561.0
  • Pubmed link: Link

  • Full research link: Link

  • Abstract:Denitrification was measured in three structurally different coastal microbial mats by using the stable isotope technique. The composition of the denitrifying community was determined by analyzing the nitrite reductase (nirS and nirK) genes using clone libraries and the GeoChip. The highest potential rate of denitrification (7.0 ± 1.0 mmol N m(-2) d(-1)) was observed during summer at station 1 (supra-littoral). The rates of denitrification were much lower in the stations 2 (marine) and 3 (intermediate) (respectively 0.1 ± 0.05 and 0.7 ± 0.2 mmol N m(-2) d(-1)) and showed less seasonality when compared to station 1. The denitrifying community at station 1 was also more diverse than that at station 2 and 3, which were more similar to each other than either of these stations to station 1. In all three stations, the diversity of both nirS and nirK denitrifiers was higher in summer when compared to winter. The location along the tidal gradient seems to determine the composition, diversity and activity of the denitrifier community, which may be driven by salinity, nitrate/nitrite and organic carbon. Both nirS and nirK denitrifiers are equally present and therefore they are likely to play a role in the denitrification of the microbial mats studied.