Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Uncultured bacterium

Taxonomy
  • Phylum : nan
  • Class : nan
  • Order : nan
  • Family : nan
  • Genus : nan

Isolation Source

Coastal wetland sediment

Enzyme Name

nitrite reductase
  • Encoding Gene:NirS
  • DNA Size：871 bp
  • Nucleotide FASTA sequence： Link

  • UniProt I.D: nan

Protein Information
  • Pro_GenBank I.D： ANQ67646.1

  • Length：290 aa
  • Protein FASTA_sequence: Link

Information about Article
  • Reference：Gao et al., 2016
  • Title：nirS-Encoding denitrifier community composition, distribution, and abundance along the coastal wetlands of China.
  • Pubmed ID：27311565.0
  • Pubmed link： Link

  • Full research link： Link

  • Abstract：For the past few decades, human activities have intensively increased the reactive nitrogen enrichment in China's coastal wetlands. Although denitrification is a critical pathway of nitrogen removal, the understanding of denitrifier community dynamics driving denitrification remains limited in the coastal wetlands. In this study, the diversity, abundance, and community composition of nirS-encoding denitrifiers were analyzed to reveal their variations in China's coastal wetlands. Diverse nirS sequences were obtained and more than 98 % of them shared considerable phylogenetic similarity with sequences obtained from aquatic systems (marine/estuarine/coastal sediments and hypoxia sea water). Clone library analysis revealed that the distribution and composition of nirS-harboring denitrifiers had a significant latitudinal differentiation, but without a seasonal shift. Canonical correspondence analysis showed that the community structure of nirS-encoding denitrifiers was significantly related to temperature and ammonium concentration. The nirS gene abundance ranged from 4.3 × 10(5) to 3.7 × 10(7) copies g(-1) dry sediment, with a significant spatial heterogeneity. Among all detected environmental factors, temperature was a key factor affecting not only the nirS gene abundance but also the community structure of nirS-type denitrifiers. Overall, this study significantly enhances our understanding of the structure and dynamics of denitrifying communities in the coastal wetlands of China.

Biological Nitrogen Removal Database