Biological Nitrogen Removal Database

Fusarium sp. OTU095 AN-2016

• Phylum : Ascomycota
• Class : Sordariomycetes
• Order : Hypocreales
• Family : Nectriaceae
• Genus : Fusarium

Soil

cytochrome P450 nitric oxide reductase 

• Encoding Gene:p450nor
• DNA Size：617 bp
• Nucleotide FASTA sequence： Link

• UniProt I.D: A0A142KX56

• Pro_GenBank I.D： AMS24532.1

• Length：154 aa
• Protein FASTA_sequence: Link

• Reference：Novinscak et al., 2016
• Title：Novel P450nor Gene Detection Assay Used To Characterize the Prevalence and Diversity of Soil Fungal Denitrifiers
• Pubmed ID：27208113
• Pubmed link： Link

• Full research link： Link

• Abstract：Denitrifying fungi produce nitrous oxide (N2O), a potent greenhouse gas, as they generally lack the ability to convert N2O to dinitrogen. Contrary to the case for bacterial denitrifiers, the prevalence and diversity of denitrifying fungi found in the environment are not well characterized. In this study, denitrifying fungi were isolated from various soil ecosystems, and novel PCR primers targeting the P450nor gene, encoding the enzyme responsible for the conversion of nitric oxide to N2O, were developed, validated, and used to study the diversity of cultivable fungal denitrifiers. This PCR assay was also used to detect P450nor genes directly from environmental soil samples. Fungal denitrification capabilities were further validated using an N2O gas detection assay and a PCR assay targeting the nirK gene. A collection of 492 facultative anaerobic fungi was isolated from 15 soil ecosystems and taxonomically identified by sequencing the internal transcribed spacer sequence. Twenty-seven fungal denitrifiers belonging to 10 genera had the P450nor and the nirK genes and produced N2O from nitrite. N2O production is reported in strains not commonly known as denitrifiers, such as Byssochlamys nivea, Volutella ciliata, Chloridium spp., and Trichocladium spp. The prevalence of fungal denitrifiers did not follow a soil ecosystem distribution; however, a higher diversity was observed in compost and agricultural soils. The phylogenetic trees constructed using partial P450nor and nirK gene sequences revealed that both genes clustered taxonomically closely related strains together.