Biological Nitrogen Removal Database

Nitrospira sp. GaII

• Phylum : Nitrospirae
• Class : nan
• Order :Nitrospirales
• Family : Nitrospiraceae
• Genus : Nitrospira

Oxygen

Sodium nitrite

Reference:Lebedeva et al., 2005

Title:Moderately thermophilic nitrifying bacteria from a hot spring of the Baikal rift zone

Pubmed ID:16332328

Pubmed link:Link

Full research link:Link

Abstract: Samples from three hot springs (Alla, Seya and Garga) located in the northeastern part of Baikal rift zone (Buryat Republic, Russia) were screened for the presence of thermophilic nitrifying bacteria. Enrichment cultures were obtained solely from the Garga spring characterized by slightly alkaline water (pH 7.9) and an outlet temperature of 75 degrees C. The enrichment cultures of the ammonia- and nitrite oxidizers grew at temperature ranges of 27-55 and 40-60 degrees C, respectively. The temperature optimum was approximately 50 degrees C for both groups and thus they can be designated as moderate thermophiles. Ammonia oxidizers were identified with classical and immunological techniques. Representatives of the genus Nitrosomonas and Nitrosospira-like bacteria with characteristic vibroid morphology were detected. The latter were characterized by an enlarged periplasmic space, which has not been previously observed in ammonia oxidizers. Electron microscopy, denaturing gradient gel electrophoresis analyses and partial 16S rRNA gene sequencing provided evidence that the nitrite oxidizers were members of the genus Nitrospira.