Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Comamonas denitrificans strain 5.38g

Taxonomy
  • Phylum : Proteobacteria
  • Class : Betaproteobacteria
  • Order :Burkholderiales
  • Family : Comamonadaceae
  • Genus : Comamonas

Electron Acceptor

NO3-

Electron Donor

Various compounds

Information about Article

Reference:Gumaelius et al., 2001

Title:Comamonas Denitrificans Sp. Nov., an Efficient Denitrifying Bacterium Isolated From Activated Sludge

Pubmed ID:11411726

Pubmed link:Link

Full research link:Link

Abstract: To find a biomarker for denitrification in activated sludge, five denitrifying strains isolated from three wastewater treatment plants were studied. These strains were selected from among 1,500 isolates for their excellent denitrifying properties. They denitrify quickly and have no lag phase when switching from aerobic to anoxic conditions. All strains have the cd1-type of nitrite reductase. The strains are Gram-negative rods and they all grow as filamentous chains when cultivated in liquid solution. The strains differ in colony morphology when grown on nutrient agar. Almost full-length 16S rDNA sequences were determined and phylogenetic analysis revealed that these strains are positioned among members of the genus Comamonas in the beta-subclass of the Proteobacteria. Signature nucleotides and bootstrap percentages were also analysed to verify this position. Strains 110, 123T, 2.99g, 5.38g and P17 were < or = 96.7% similar to known strains, but > or = 99.7% similar to each other, as judged from their 16S rDNA sequences, and grouped tightly together in the phylogenetic tree. Sequence motifs in the 16S rRNA gene were also found, suggesting the monophyletic origin of these strains. Nevertheless, some strains differed from the others, for example strain 110 branches early from the other strains and 5.38g is phenotypically more inert. Therefore, it is proposed that strains 110, 123T, 2.99g and P17 are classified into a new species, Comamonas denitrificans sp. nov., while the taxonomic status of strain 5.38g will have to await the outcome of further studies. The type strain of Comamonas denitrificans is 123T (ATCC 700936T).

Biological Nitrogen Removal Database