Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Pseudomonas stutzeri strain HD4-1

Taxonomy
  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order :Pseudomonadales
  • Family : Pseudomonadaceae
  • Genus : Pseudomonas

Electron Acceptor

NO3-

Electron Donor

Succinate, citrate, and acetate

Information about Article

Reference:Zheng et al., 2014

Title:Reducing NO and N₂O Emission During Aerobic Denitrification by Newly Isolated Pseudomonas Stutzeri PCN-1

Pubmed ID:24747385

Pubmed link:Link

Full research link:Link

Abstract: As two obligatory intermediates of denitrification, both NO and N2O had harmful environmental and biological impacts. An aerobic denitrifying bacterial strain PCN-1 was newly isolated and identified as Pseudomonas stutzeri, which was capable of high efficient nitrogen removal under aerobic condition with maximal NO and N2O accumulation as low as 0.003% and 0.33% of removed NO3(-)-N, respectively. Further experiment taking nitrite as denitrifying substrate indicated similar low NO and N2O emission of 0.006% and 0.29% of reduced NO2(-)-N, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the coordinate expression of denitrification gene nirS (for cytochrome cd1 nitrite reductase), cnorB (for NO reductase) and nosZ (for N2O reductase) was the fundamental reason of low NO and N2O accumulation. Activated sludge system bioaugmented by strain PCN-1 demonstrated a significant reduction of NO and N2O emission from wastewater during aerobic denitrification, implied great potential of PCN-1 in practical applications.

Biological Nitrogen Removal Database