Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Thauera sp. strain SND5

Taxonomy

  • Phylum : Proteobacteria
  • Class : Betaproteobacteria
  • Order :Rhodocyclales
  • Family : Zoogloeaceae
  • Genus : Thauera

Electron Acceptor

Oxygen; Polyhydroxybutyrate (PHB

Electron Donor

Ammonium sulfate (NH4)2SO4

Information about Article

Reference:Wang et al., 2020

Title:Complete nitrogen removal via simultaneous nitrification and denitrification by a novel phosphate accumulating Thauera sp. strain SND5

Pubmed ID:32823196.0

Pubmed link:Link

Full research link:Link

Abstract: Bacteria capable of simultaneous nitrification and denitrification (SND) and phosphate removal could eliminate the need for separate reactors to remove nutrients from wastewater and alleviate competition for carbon sources between different heterotrophs in wastewater treatment plants (WWTPs). Here we report a newly isolated Thauera sp. strain SND5, that removes nitrogen and phosphorus from wastewater via SND and denitrifying-phosphate accumulation, respectively, without accumulation of metabolic intermediates. Strain SND5 simultaneously removes ammonium, nitrite, and nitrate at an average rate of 2.85, 1.98, and 2.42 mg-N/L/h, respectively. Batch testing, detection of functional genes, nitrogenous gas detection and thermodynamic analysis suggested that nitrogen gas, with hydroxylamine produced as an intermediate, was the most likely end products of heterotrophic ammonium oxidation by strain SND5. The generated end products and intermediates suggest a novel nitrogen removal mechanism for heterotrophic ammonium oxidation in strain SND5 (NH4+→NH2OH→N2). Strain SND5 was also found to be a denitrifying phosphate-accumulating organism, capable of accumulating phosphate, producing and storing polyhydroxybutyrate (PHB) as an intracellular source of carbon while using nitrate/nitrite or oxygen as an electron acceptor during PHB catabolism. This study identifies a novel pathway by which simultaneous nitrogen and phosphorus removal occurs in WWTPs via a single microbe.