Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Sequencing batch reactor (SBR) reactor
Medium:Granular ANAMMOX sludge
Culture taken from:Methanogenic anaerobic granular sludge
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:Ammonium chloride NH4Cl
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.5–8.3
Maximum sludge concentration:7.095
HRT:3 d
NH4–N Influent conc(mg/L):268
NO2–N Influent conc(mg/L):345
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):83.6
NO2–N Removal efficiency (%):100
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:nan
NRR kg-N/m3/d:nan
Major findings:Anaerobic methanogenic granular sludge was a very suitable inoculum for the enrichment of anammox bacteria. The SBR is a suitable system for the enrichment of a microbial community with an extremely slow growth rate.
Authors:Liao et al., 2008
Title:Effect of inorganic carbon on anaerobic ammonium oxidation enriched in sequencing batch reactor
Pubmed link:Link
Full research link:Link
Abstract:The present lab-scale research reveals the enrichment of anaerobic ammonium oxidation microorganism from methanogenic anaerobic granular sludge and the effect of inorganic carbon (sodium bicarbonate) on anaerobic ammonium oxidation. The enrichment of anammox bacteria was carried out in a 7.0-L sequencing batch reactor (SBR) and the effect of bicarbonate on anammox was conducted in a 3.0-L SBR. Research results, especially the biomass, showed first signs of anammox activity after 54 d cultivation with synthetic wastewater, when the pH was controlled between 7.5 and 8.3, the temperature was 35 degrees C. The anammox activity increased as the influent bicarbonate concentration increased from 1.0 to 1.5 g/L, and then, was inhibited as the bicarbonate concentration approached 2.0 g/L. However, the activity could be restored by the reduction of bicarbonate concentration to 1.0 g/L, as shown by rapid conversion of ammonium, and nitrite and nitrate production with normal stoichiometry. The optimization of the bicarbonate concentration in the reactor could increase the anammox rate up to 66.4 mgN/(L x d).