Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Anammox


Experimental setup


Influent:Slurry-type piggery waste

Anammox system:nan

Anammox reactor:Up Flow - Anaerobic Sludge Blanket Reactor (UASB) reactor

Medium:Granular sludge

Culture taken from:Granular sludge from a full-scale UASB reactor, treating brewery wastewater

Microorganism cultured:nan

Respiration:Anaerobic

Electron donor:Ammonium chloride NH4Cl

Electron acceptor:Sodium Nitrite (NaNO2)

PH:8.4–8.6

Maximum sludge concentration:80

HRT:5 d

NH4–N Influent conc(mg/L):570

NO2–N Influent conc(mg/L):nan

SO4–S Influent conc(mg/L):nan


Experimental Information


NH4–N Removal efficiency (%):74

NO2–N Removal efficiency (%):71

SO4-S Removal efficiency (%):nan

NLR kg-N/m3/d:0.43

NRR kg-N/m3/d:nan


Information about Article


Major findings:The result of this research demonstrates that, in the UASB reactor, anaerobic ammonium removal from strong nitrogenous waste, such as piggery waste, can be performed successfully. Furthermore, it appears that by using granular sludge as the seed biomass, the ANAMMOX reaction can start more quickly, implying that a UASB reactor has many advantages such as an easy start-up and operation

Authors:Ahn et al., 2004

Title:ANAMMOX and partial denitritation in anaerobic nitrogen removal from piggery waste

Pubmed link:Link

Full research link:Link

Abstract:The anaerobic ammonium removal from a piggery waste with high strength (56 g COD/L and 5 g T-N/L) was investigated using a lab-scale upflow anaerobic sludge bed reactor at a mesophilic condition. Based on the nitrogen and carbon balance in the process, the contribution of autotrophic and heterotrophic organisms was also evaluated in terms of the influent NO2-N/NH4-N ratio (1:0.8 and 1:1.2 for Phase 1 and Phase 2, respectively). The result of this research demonstrates that the anaerobic ammonium removal from the piggery waste, using the UASB reactor, can be performed successfully. Furthermore, it appears that by using granular sludge as the seed biomass, the ANAMMOX reaction can start more quickly. Average nitrogen conversion was 0.59 kg T-N/m3 reactor-day (0.06 kg T-N/kg VSS/day) and 0.66 kg T-N/m3 reactor-day (0.08 kg T-N/kg VSS/day) for Phase 1 and Phase 2. The NO2-N/NH4-N removal ratio by the ANAMMOX was 1.48 and 1.79 for Phase 1 and Phase 2. The higher nitrite contents (about 50%) in the substrate resulted in higher nitrite nitrogen removal by the partial denitritation, as well as the ANAMMOX reaction, implying higher potential of partial denitritation. However, the result reveals that the ANAMMOX reaction was influenced less by the degree of partial denitritation, and the ANAMMOX bacteria did not compete with denitritation bacteria. The colour of the biomass at the bottom of the reactor changed from dark gray to dark red, which was accompanied by an increase in cytochrome content. At the end of the experiment, red-coloured granular sludge with diameter of 1-2 mm at the lower part of the reactor was also observed.