Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Waste brine
Anammox system:nan
Anammox reactor:Up Flow - Anaerobic Sludge Blanket Reactor (UASB) reactor
Medium:Methanogenic granule
Culture taken from:Methanogenic granule biomass
Microorganism cultured:Candidatus Scalindua wagneri, Methanosaeta
Respiration:Anaerobic
Electron donor:Ammonium chloride NH4Cl
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.5
Maximum sludge concentration:18.9
HRT:27.3 h
NH4–N Influent conc(mg/L):180
NO2–N Influent conc(mg/L):180
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):70
NO2–N Removal efficiency (%):70
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:0.37
NRR kg-N/m3/d:10.7
Major findings:The success of enriching anammox bacteria amenable to waste brine with high salinity could lead to development of a PN-anammox process. This study demonstrated a robust startup strategy of an anammox reactor to treat waste brine containing high concentrations of nitrogen and salt.
Authors:Yokota et al., 2018
Title:High-rate nitrogen removal from waste brine by marine anammox bacteria in a pilot-scale UASB reactor
Pubmed link:Link
Full research link:Link
Abstract:The goal of this study was to develop a startup strategy for a high-rate anaerobic ammonium oxidation (anammox) reactor to treat waste brine with high concentrations of ammonium from a natural gas plant. An upflow anaerobic sludge blanket (UASB) anammox reactor with an effective volume of 294 L was fed continuously with waste brine with a salinity of 3% and a NH4+ concentration of 180 mg-N/L, as well as a NaNO2 solution. By inoculating a methanogenic granular biomass as a biomass carrier, the reactor attained the maximum volumetric nitrogen removal rate (NRR) of 10.7 kg-N/m3/day on day 209, which was 1.7 times higher than the highest reported NRR for wastewater of comparable salinity. High-throughput sequencing of 16S rRNA gene amplicons revealed that Candidatus Scalindua wagneri was enriched successfully in granules in the UASB, and it replaced Methanosaeta and became dominant in the granule. The inhibitory effect of NO2- on the anammox reaction in the granules was assessed by a 15N tracer method, and the results showed that anammox activity was maintained at 60% after exposure to 300 mg-N/L of NO2- for 24 h. Compared with previous studies of the susceptibilities of Candidatus Brocadia and Candidatus Kuenenia to NO2-, the enriched marine anammox bacteria were proven to have comparable or even higher tolerances for high NO2- concentrations after a long exposure.