Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Sequencing batch reactor (SBR)
Medium:Granular sludge
Culture taken from:Denitrifying ± and anaerobic ammoniumoxidizing ± fluidized bed reactor
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:Ammonium sulfate ((NH4)2SO4)
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.0–8.0
Maximum sludge concentration:nan
HRT:nan
NH4–N Influent conc(mg/L):nan
NO2–N Influent conc(mg/L):nan
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):nan
NO2–N Removal efficiency (%):nan
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:1
NRR kg-N/m3/d:nan
Major findings:This study has shown that reliable, convenient techniques such as the SBR can lead to the enrichment and study of organisms that may be out of reach for classical microbiological techniques.
Authors:Strous et al., 1998
Title:The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms
Pubmed link:None
Full research link:Link
Abstract:Currently available microbiological techniques are not designed to deal with very slowly growing microorganisms. The enrichment and study of such organisms demands a novel experimental approach. In the present investigation, the sequencing batch reactor (SBR) was applied and optimized for the enrichment and quantitative study of a very slowly growing microbial community which oxidizes ammonium anaerobically. The SBR was shown to be a powerful experimental set-up with the following strong points: (1) efficient biomass retention, (2) a homogeneous distribution of substrates, products and biomass aggregates over the reactor, (3) reliable operation for more than 1 year, and (4) stable conditions under substrate-limiting conditions. Together, these points made possible for the first time the determination of several important physiological parameters such as the biomass yield (0.066?±?0.01 C-mol/mol ammonium), the maximum specific ammonium consumption rate (45?±?5?nmol/mg protein/min) and the maximum specific growth rate (0.0027?·?h?1, doubling time 11 days). In addition, the persisting stable and strongly selective conditions of the SBR led to a high degree of enrichment (74% of the desired microorganism). This study has demonstrated that the SBR is a powerful tool compared to other techniques used in the past. We suggest that the SBR could be used for the enrichment and quantitative study of a large number of slowly growing microorganisms that are currently out of reach for microbiological research.