Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Sequencing batch reactor (SBR)
Medium:nan
Culture taken from:nan
Microorganism cultured:Brocadia anammoxidans, Brocadia fulgida, Brocadia sp., and “Candidatus Kuenenia”
Respiration:Anaerobic
Electron donor:Ammonium sulfate ((NH4)2SO4)
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.5–8.5
Maximum sludge concentration:0.4
HRT:nan
NH4–N Influent conc(mg/L):247
NO2–N Influent conc(mg/L):248
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):nan
NO2–N Removal efficiency (%):nan
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:580
NRR kg-N/m3/d:0.25^
Major findings:The microbial community structure changed during the Nremoval recovery process, from a very diverse microbial community just after the temperature shock, to an anammox enriched community in the fully recovered reactor.
Authors:Isanta et al., 2015
Title:Microbial community shifts on an anammox reactor after a temperature shock using 454-pyrosequencing analysis
Pubmed link:Link
Full research link:Link
Abstract:To explore the changes in the microbial community structure during the recovery process of an anammox reactor after a temperature shock, the 454-pyrosequencing technique was used. The temperature shock reduced the nitrogen removal rate up to 92% compared to that just before the temperature shock, and it took 70 days to recover a similar nitrogen removal rate to that before the temperature shock (ca. 0.30 g N L(-1) d(-1)). Pyrosequencing results indicated that microbial diversity in the reactor decreased as the reactor progressively recovered from the temperature shock. Anammox bacteria were accounted as 6%, 35% and 46% of total sequence reads in samples taken 13, 45 and 166 days after the temperature shock. These results were in agreement with N-removal performance results and anammox activity measured in the reactor during the recovery process. An anammox specific primer was used to precisely determine the anammox species in the biomass samples.