Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Single PN/A up flow reactor
Medium:Non-woven fabric support
Culture taken from:Municipal wastewater treatment plant
Microorganism cultured:Candidatus Brocadia anammoxidans
Respiration:Anaerobic
Electron donor:Ammonium sulfate ((NH4)2SO4)
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.86
Maximum sludge concentration:nan
HRT:1–5 h
NH4–N Influent conc(mg/L):206
NO2–N Influent conc(mg/L):206
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):16.6
NO2–N Removal efficiency (%):22.1
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:3.6
NRR kg-N/m3/d:0.35
Major findings:Simultaneous partial nitrification and anammox reactions were successfully established in single up-flow oxygen-limiting biofilm reactors originally started as either a partial nitrifying reactor or an anammox reactor, without external biomass inoculation
Authors:Cho et al., 2011
Title:Development of a simultaneous partial nitrification and anaerobic ammonia oxidation process in a single reactor
Pubmed link:Link
Full research link:Link
Abstract:Up-flow oxygen-controlled biofilm reactors equipped with a non-woven fabric support were used as a single reactor system for autotrophic nitrogen removal based on a combined partial nitrification and anaerobic ammonium oxidation (anammox) reaction. The up-flow biofilm reactors were initiated as either a partial nitrifying reactor or an anammox reactor, respectively, and simultaneous partial nitrification and anammox was established by careful control of the aeration rate. The combined partial nitrification and anammox reaction was successfully developed in both biofilm reactors without additional biomass inoculation. The reactor initiated as the anammox reactor gave a slightly higher and more stable mean nitrogen removal rate of 0.35 (±0.19) kg-N m(-3) d(-1) than the reactor initiated as the partial nitrifying reactor (0.23 (±0.16) kg-N m(-3) d(-1)). FISH analysis revealed that the biofilm in the reactor started as the anammox reactor were composed of anammox bacteria located in inner anoxic layers that were surrounded by surface aerobic AOB layers, whereas AOB and anammox bacteria were mixed without a distinguishable niche in the biofilm in the reactor started as the partial nitrifying reactor. However, it was difficult to efficiently maintain the stable partial nitrification owing to inefficient aeration in the reactor, which is a key to development of the combined partial nitrification and anammox reaction in a single biofilm reactor.