Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Synthetic wastewate

Anammox system:nan

Anammox reactor:Upflow anaerobic filter system

Medium:Three-dimensional-plastic media

Culture taken from:Activated sludge taken from a mesophilic digester of a municipal wastewater treatment plant

Microorganism cultured:nan


Electron donor:Ammonium sulfate ((NH4)2SO4)

Electron acceptor:Sodium Nitrite (NaNO2)


Maximum sludge concentration:46.1

HRT:1.99 h

NH4–N Influent conc(mg/L):305

NO2–N Influent conc(mg/L):304

SO4–S Influent conc(mg/L):nan

Experimental Information

NH4–N Removal efficiency (%):74.2

NO2–N Removal efficiency (%):92.4

SO4-S Removal efficiency (%):nan

NLR kg-N/m3/d:7.34

NRR kg-N/m3/d:6.11

Information about Article

Major findings:Substrate removal efficiencies ranged from 71.5% to 98.2% during the experimental studies

Authors:Jin et al., 2009

Title:Kinetics of nitrogen removal in high rate anammox upflow filter

Pubmed link:Link

Full research link:Link

Abstract:The process kinetics for laboratory-scale anammox (anaerobic ammonium oxidation) upflow filter using synthetic wastewater as feed were investigated. The experimental unit consisted of a 2.0 L reactor filled with three-dimensional plastic media. The filter was tested for different influent substrate concentrations and hydraulic retention time (HRT). The substrate loading removal rate was compared with prediction of Stover-Kincannon, second-order and the first-order substrate removal models. Upon approaching pseudo-steady-state condition, substrate ammonium or nitrite concentrations were increased from 280 to 462 mg N/L, while HRT was stepwise decreased from 14.4 to 2h, with a concomitant increase in nitrogen loading rate (NLR) from 0.93 to 7.34 g/L day. Based on calculations, Stover-Kincannon model and second-order "Grau" model were found to be the appropriate models to describe the upflow filter. According to Stover-Kincannon model, the maximum total substrate removal rate constant (U(max)) and saturation value constant (K(B)) were suggested as 12.4 and 12.0 g N/L day, respectively. As Stover-Kincannon model and second-order model gave high correlation coefficients (97.9% and 98.6%, respectively), these models may be used in predicting the behavior or design of the anammox filter.