Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Sequencing batch reactor (SBR)
Medium:Granular sludge
Culture taken from:Domestic wastewater treatment plant
Microorganism cultured:Nitrobacter sp., Nitrospira sp.
Respiration:Anaerobic
Electron donor:Ammonium sulfate ((NH4)2SO4)
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7.5
Maximum sludge concentration:0.37
HRT:1 d
NH4–N Influent conc(mg/L):57
NO2–N Influent conc(mg/L):57
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):48
NO2–N Removal efficiency (%):48
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:nan
NRR kg-N/m3/d:0.6
Major findings:A closed-loop concept for the operation of a WWTP with the FNA-based method was developed in this study. Increasing O2 from 2 to 8 mg per litre decreased the nitrogen removal rate
Authors:Wang et al., 2016
Title:Achieving Stable Nitritation for Mainstream Deammonification by Combining Free Nitrous Acid-Based Sludge Treatment and Oxygen Limitation
Pubmed link:None
Full research link:Link
Abstract:Stable nitritation is a critical bottleneck for achieving autotrophic nitrogen removal using the energy-saving mainstream deammonification process. Herein we report a new strategy to wash out both the Nitrospira sp. and Nitrobacter sp. from the treatment of domestic-strength wastewater. The strategy combines sludge treatment using free nitrous acid (FNA) with dissolved oxygen (DO) control in the nitritation reactor. Initially, the nitrifying reactor achieved full conversion of NH4+ to NO3?. Then, nitrite accumulation at ~60% was achieved in the reactor when 1/4 of the sludge was treated daily with FNA at 1.82?mg N/L in a side-stream unit for 24?h. Fluorescence in-situ hybridization (FISH) revealed FNA treatment substantially reduced the abundance of nitrite oxidizing bacteria (NOB) (from 23.0?±?4.3 to 5.3?±?1.9%), especially that of Nitrospira sp. (from 15.7?±?3.9 to 0.4?±?0.1%). Nitrite accumulation increased to ~80% when the DO concentration in the mainstream reactor was reduced from 2.5–3.0 to 0.3–0.8?mg/L. FISH revealed the DO limitation further reduced the abundance of NOB (to 2.1?±?1.0%), especially that of Nitrobacter sp. (from 4.9?±?1.2 to 1.8?±?0.8%). The strategy developed removes a major barrier for deammonification in low-strength domestic wastewater.