Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Anammox system:nan
Anammox reactor:Membrane biofilm reactor (MBFR)
Medium:Non-porous 116 polypropylene hollow-fiber
Culture taken from:Parent reactor consisting of 133 anammox and DAMO microorganisms
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:Ammonium chloride NH4Cl
Electron acceptor:Sodium Nitrite (NaNO2)
PH:7
Maximum sludge concentration:nan
HRT:4–12 h
NH4–N Influent conc(mg/L):130
NO2–N Influent conc(mg/L):25
SO4–S Influent conc(mg/L):nan
NH4–N Removal efficiency (%):94.7
NO2–N Removal efficiency (%):nan
SO4-S Removal efficiency (%):nan
NLR kg-N/m3/d:0.1–0.31
NRR kg-N/m3/d:0.2
Major findings:High effluent nitrogen in the system causes unsatisfactory nutrient ratio 1.32:1 (nitrite to ammonium) at 20C
Authors:Xie et al., 2018
Title:Achieving high-level nitrogen removal in mainstream by coupling anammox with denitrifying anaerobic methane oxidation in a membrane biofilm reactor
Pubmed link:Link
Full research link:Link
Abstract:To achieve energy neutral wastewater treatment, mainstream anaerobic ammonium oxidation (anammox) has attracted extensive attention in the past decade. However, the relatively high effluent nitrogen concentration (>10 mg N L-1) remains a significant barrier hindering its practical implementation. A novel technology integrating the anammox and denitrifying anaerobic methane oxidation (DAMO) reactions in a membrane biofilm reactor (MBfR) was developed in this study to enhance the mainstream anammox process. With the hydraulic retention time (HRT) progressively decreased from 12 to 4 h, the total nitrogen (TN) removal rate increased stepwise from 0.09 to 0.28 kg N m-3 d-1, with an effluent TN concentration below 3.0 mg N L-1 achieved. Mass balance analysis showed that 30-60% of the nitrate produced by the anammox reaction was reduced back to nitrite by DAMO archaea, and the anammox and DAMO bacteria were jointly responsible for nitrite removal with contributions of >90% and <10%, respectively. Additionally, the established MBfR was robust and achieved consistently high effluent quality with >90% TN removal when the influent nitrite to ammonium molar ratio varied in the range of 1.17-1.55. Fluorescence in situ hybridization (FISH) and 16S rRNA gene sequencing indicated that anammox bacteria, DAMO bacteria and DAMO archaea jointly dominated the biofilm, and were likely the key contributors to nitrogen removal. This is the first study that a high nitrogen removal rate (>0.2 kg N m-3 d-1) and satisfactory effluent quality (?3 mg TN L-1) were achieved simultaneously by integrating anammox and DAMO reactions in mainstream wastewater treatment.