Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Comammox System:MBfR coupling anammox and n-DAMO
reactor:Lab scale membrane biofilm reactor
Medium:Biofilm-suspended-growth
Culture taken from:Anammox and n-DAMO co-culture in an MBfR
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:Methane
Electron acceptor:Nitrite
PH:7-7.5
Temperature:10–25°C
HRT:9 h
NH4–N Influent conc(mg/L):30
NO2–N Influent conc(mg/L):22
NO3–N Influent conc(mg/L):nan
NH4–N Effluent (mg N/L):<1
NO2–N Effluent (mg N/L):<1
NO3-N Effluent (mg N/L):4
NH4–N removal rate mg/L/d:nan
NO2–N removal rate mg/L/d:nan
NO3-N removal rate mg/L/d:nan
TN Removal rate (mg N/L/d):130.0
Authors:Liu et al., 2014
Title:Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor.
Pubmed link:None
Full research link:Link
Abstract:The mainstream anaerobic ammonium oxidation (anammox) process provides strong support to the on-going paradigm shift from energy-negative to energy-neutral in wastewater treatment plants. However, the low temperature (e.g., below 15 °C) represents one of the major challenges for mainstream anammox in practice. In this study, a stable nitrogen removal rate (0.13 kg m–3 day–1), together with a high-level effluent quality (<5.0 mg N L–1), was achieved in a lab-scale upflow membrane biofilm reactor (MBfR) by coupling anammox with nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) microorganisms, at a temperature as low as 10 °C. With the temperature being progressively decreased from 25 to 10 °C, the total nitrogen removal efficiency was maintained in the range of 90–94% at a constant hydraulic retention time of 9 h. The impact of temperature on the biofilm system coupling anammox and n-DAMO reactions increased at a lower temperature range with higher Arrhenius coefficients. Additionally, 16S rRNA gene sequencing results showed that anammox bacteria, n-DAMO bacteria, and n-DAMO archaea jointly dominated the biofilm, and their respective abundances remained relatively stable when the temperature was decreased. The major reason for this temperature-tolerated performance is the overcapacity developed, which is indicated by biofilm thickness measurements and mathematical modeling. The stable performance obtained in this study shows promise for the n-DAMO application in domestic wastewater.