Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Real wastewater
Comammox System:Sequential batch reactor coupling anammox and n-DAMO
reactor:Sequential batch reactor (SBR)
Medium:Suspended-sludge
Culture taken from:Enriched n-DAMO archaea and anammox bacteria culture
Microorganism cultured:n-DAMO archaea and n-DAMO bacteria
Respiration:Anaerobic
Electron donor:Methane
Electron acceptor:Nitrite
PH:7.0–7.5
Temperature:35°C
HRT:nan
NH4–N Influent conc(mg/L):6?mM
NO2–N Influent conc(mg/L):6?mM
NO3–N Influent conc(mg/L):nan
NH4–N Effluent (mg N/L):nan
NO2–N Effluent (mg N/L):nan
NO3-N Effluent (mg N/L):nan
NH4–N removal rate mg/L/d:11.6-16.6
NO2–N removal rate mg/L/d:21.9
NO3-N removal rate mg/L/d:4.4-13
TN Removal rate (mg N/L/d):nan
Authors:Raghoebarsing et al., 2006
Title:A microbial consortium couples anaerobic methane oxidation to denitrification
Pubmed link:None
Full research link:Link
Abstract:Modern agriculture has accelerated biological methane and nitrogen cycling on a global scale1,2. Freshwater sediments often receive increased downward fluxes of nitrate from agricultural runoff and upward fluxes of methane generated by anaerobic decomposition3. In theory, prokaryotes should be capable of using nitrate to oxidize methane anaerobically, but such organisms have neither been observed in nature nor isolated in the laboratory4,5,6,7,8. Microbial oxidation of methane is thus believed to proceed only with oxygen or sulphate9,10. Here we show that the direct, anaerobic oxidation of methane coupled to denitrification of nitrate is possible. A microbial consortium, enriched from anoxic sediments, oxidized methane to carbon dioxide coupled to denitrification in the complete absence of oxygen. This consortium consisted of two microorganisms, a bacterium representing a phylum without any cultured species and an archaeon distantly related to marine methanotrophic Archaea. The detection of relatives of these prokaryotes in different freshwater ecosystems worldwide11,12,13,14 indicates that the reaction presented here may make a substantial contribution to biological methane and nitrogen cycles.