Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Real wastewater

Comammox System:MBfR coupling anammox and n-DAMO

reactor:Lab scale membrane biofilm reactor


Culture taken from:Anammox and DAMO microorganisms from sewage treatment plant

Microorganism cultured:n-DAMO archaea and n-DAMO bacteria


Electron donor:Methane

Electron acceptor:Nitrite



HRT:24 h

NH4–N Influent conc(mg/L):12

NO2–N Influent conc(mg/L):62

NO3–N Influent conc(mg/L): 1-22

Experimental Information

NH4–N Effluent (mg N/L):<1

NO2–N Effluent (mg N/L):<1

NO3-N Effluent (mg N/L):<1

NH4–N removal rate mg/L/d:nan

NO2–N removal rate mg/L/d:nan

NO3-N removal rate mg/L/d:nan

TN Removal rate (mg N/L/d):98.0

Information about Article

Authors:Lim et al., 2021

Title:Versatility of nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO): First demonstration with real wastewater

Pubmed link:None

Full research link:Link

Abstract:Nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) processes have been proven effective for nitrogen removal from synthetic wastewater. However, the demonstration using real wastewater has not been achieved yet. To this end, this study investigated the versatile applications of n-DAMO process in real wastewater treatment for the first time. Two methane-based membrane biofilm reactors (MBfRs) were employed to combine anammox and n-DAMO microorganisms, targeting nitrogen removal in mainstream (i.e., domestic sewage) and sidestream (i.e., anaerobic digestion liquor), respectively. Considering various technologies in sewage treatment, three different technical routes, including nitritation + methane-based MBfR, partial nitritation + methane-based MBfR and partial nitritation + anammox + methane-based MBfR, were investigated comprehensively, all producing effluent quality with total nitrogen (TN) at 5 mg N/L or less. Regarding the sidestream treatment, the methane-based MBfR also removed up to 96% TN from the partially nitrified anaerobic digestion liquor at a practically useful rate of 0.5 kg N/m3/d. Microbial communities revealed by 16S rRNA gene amplicon sequencing indicated the dominance of n-DAMO archaea in both reactors, along with the existence of anammox bacteria and n-DAMO bacteria. As the first demonstration of n-DAMO process in real wastewater, this study comprehensively confirmed the applicability of using methane as carbon source to remove nitrogen from both mainstream and sidestream wastewater, supporting their adoption by industries in practice.