Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Comammox System:MBfR coupling anammox and n-DAMO
reactor:Sequential batch reactor
Medium:Biofilm-suspended-growth
Culture taken from:Anammox and DAMO microorganisms from sewage treatment plant
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:Methane
Electron acceptor:Nitrite
PH:7
Temperature:22°C
HRT:3 days
NH4–N Influent conc(mg/L):200-300
NO2–N Influent conc(mg/L):nan
NO3–N Influent conc(mg/L):200-600
NH4–N Effluent (mg N/L):99
NO2–N Effluent (mg N/L):nan
NO3-N Effluent (mg N/L):99
NH4–N removal rate mg/L/d:nan
NO2–N removal rate mg/L/d:nan
NO3-N removal rate mg/L/d:nan
TN Removal rate (mg N/L/d):0.17
Authors:Chen et al., 2016
Title:Achieving complete nitrogen removal by coupling nitritation-anammox and methane-dependent denitrification: A model-based study
Pubmed link:None
Full research link:Link
Abstract:The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitritation-anaerobic ammonium oxidation (Anammox) and methane-dependent denitrification in membrane biofilm reactors (MBfRs). A mathematical model was applied to describe the microbial interactions among Anammox bacteria, DAMO archaea, and DAMO bacteria. The model sufficiently described the batch experimental data from an MBfR containing an Anammox-DAMO biofilm with different feeding nitrogen compositions, which confirmed the validity of the model. The effects of process parameters on the system performance and microbial community structure could therefore be reliably evaluated. The impacts of nitritation produced NO2(-)/NH4(+) ratio, methane supply, biofilm thickness and total nitrogen (TN) surface loading were comprehensively investigated with the model. Results showed that the optimum NO2(-)/NH4(+) ratio produced from nitritation for the Anammox-DAMO biofilm system was around 1.0 in order to achieve the maximum TN removal (over 99.0%), independent on TN surface loading. The corresponding optimal methane supply increased while the associated methane utilization efficiency decreased with the increase of TN surface loading. The cooperation between DAMO organisms and Anammox bacteria played the key role in the TN removal. Based on these results, the proof-of-concept feasibility of a single-stage MBfR coupling nitritation-Anammox-DAMO for complete nitrogen removal was also tested through integrating the model with ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) processes whilst controlling the dissolved oxygen (DO) concentration in the simulated system. The maximum TN removal was found to be achieved at the bulk DO concentration of around 0.17 g m(-3) under the simulation conditions, with the AOB, Anammox bacteria and DAMO organisms coexisting in the biofilm.