Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Real wastewater

Comammox System:MBfR coupling anammox and n-DAMO

reactor:Lab scale membrane biofilm reactor


Culture taken from:Anammox and DAMO microorganisms from sewage treatment plant

Microorganism cultured:n-DAMO archaea and n-DAMO bacteria


Electron donor:Methane

Electron acceptor:Nitrite



HRT:8.1 days

NH4–N Influent conc(mg/L):193.5

NO2–N Influent conc(mg/L):20

NO3–N Influent conc(mg/L):220.1 

Experimental Information

NH4–N Effluent (mg N/L):87.1

NO2–N Effluent (mg N/L):nan

NO3-N Effluent (mg N/L):127.7 

NH4–N removal rate mg/L/d:85.0 

NO2–N removal rate mg/L/d:nan

NO3-N removal rate mg/L/d:140

TN Removal rate (mg N/L/d):189.6

Information about Article

Authors:Nie et al., 2020

Title:Operation strategies of n-DAMO and Anammox process based on microbial interactions for high rate nitrogen removal from landfill leachate

Pubmed link:None

Full research link:Link

Abstract:Nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) coupling to Anaerobic ammonium oxidation (Anammox) provides an opportunity for simultaneous nitrogen removal and methane emissions mitigation from wastewater. However, to achieve high nitrogen removal rate in such a process remains a critical challenge in practical application. This work investigated the interactions between n-DAMO and Anammox in membrane biofilm reactor (MBfR) and then developed operational strategies of MBfR for high rate nitrogen removal from landfill leachate. Initially, influent containing nitrate and ammonium facilitated the development of n-DAMO and Anammox microorganisms in MBfR, but nitrogen removal performance is hard to be further improved even deteriorated. Detailed investigations of interactions among n-DAMO and Anammox microorganisms confirmed that extra addition of nitrite into MBfR fed with nitrate and ammonium not only stimulated the activities of Anammox bacteria, but also enhanced the activities of n-DAMO archaea from 172.3 to 356.9 mg NO3?-N L?1 d?1. Functional gene analysis also indicated that mcrA and hzsA genes increased after nitrite addition. Based on this finding, influent containing NO3?, NO2? and NH4+ enabled nitrogen removal rates of MBfR increase from 224.9 to 888.2 mg N L?1 d?1. Finally, nitrate in the influent was gradually replaced with nitrite to mimic the effluent from partial nitriation of landfill leachate, but maintain the nitrate availability for n-DAMO archaea through increasing nitrate production from Anammox. These operation strategies enabled MBfR achieve the steady state with a nitrogen removal rate of 6.1 kg N m?3 d?1. Microbial community analysis revealed n-DAMO archaea, n-DAMO bacteria and Anammox bacteria jointly dominated the biofilm, and their relative abundance dynamically shifted with feeding regime. This work provides promising operational strategies for high rate of nitrogen removal from landfill leachate through integrating n-DAMO and Anammox process.