Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Activated sludge
DNRA system:Dissimilatory nitrate reduction to ammonium (DNRA
reactor:Membrane bio-reactor (MBR), sequencing batch reactor (SBR)
Medium:Suspended sludge growth
Culture taken from:Wastewater, Activated sludge
Microorganism cultured:nan
Respiration:Anaerobic
Electron donor:nan
Electron acceptor:Nitrate
PH:7
Temperature:25 °C
HRT:nan
NO3–N Influent conc(mg/L):10.3
NH4–N accumulation (mg/L/d):0.4–2.1 nmol
NO2–N removal rate (mg/L/d):nan
NH4–N Accumulation rate (mg N/L/d):0.4–2.1 nmol
NH4–N Effluent (mg N/L):
NO2–N Effluent (mg N/L):
NO3-N Effluent (mg N/L):
NH4–N removal rate mg/L/d:
NO2–N removal rate mg/L/d:nan
NO3-N removal rate mg/L/d:
TN Removal rate (mg N/L/d):
Authors:Wang et al., 2020
Title:Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution
Pubmed link:None
Full research link:Link
Abstract:Recent reports on the occurrence and contribution of dissimilatory nitrate reduction to ammonium (DNRA) in marine, inland water, and soil systems have greatly improved our understanding of the global nitrogen (N) cycle. This also promoted the investigation of the role and ecological features of DNRA in anthropogenic ecosystems. However, so far, the use of DNRA in municipal wastewater treatment plants (WWTPs), which are one of the most common and largest biotechnologically artificial water ecosystems, has not been investigated. Accordingly, this study focused on the abundance, activity, community structure, and diversity of DNRA bacteria in full-scale WWTPs. DNRA bacteria were detected in all treatment units in six tested municipal WWTPs, even in aerobic zones (dissolved oxygen > 2 mg L?1). Although the relative abundance of DNRA bacteria (0.2–4.0%) was less than that of denitrifying bacteria (0.7–10.1%) among all investigated samples, the abundance of DNRA bacteria still reaches 109 gene copies g?1. However, 15N-isotope tracing indicated that the potential DNRA rates were significantly lower (0.4–2.1 nmol N g?1 h?1) than those of denitrification (9.5–15.7 nmol N g?1 h?1), but higher than anammox rate (0.3–1.3 nmol N g?1 h?1). The DNRA bacterial community structure was primarily affected by temperature gradient despite the treatment process. High-throughput sequencing analysis targeting the DNRA nrfA gene showed that Nitrospira accounted for the largest proportion of nrfA genes among all samples (6.2–36.3%), followed by Brocadia (5.9–22.1%). Network analysis further indicated that Nitrospira played an important role in both the DNRA bacterial community and entire bacterial community in municipal WWTPs. These results suggest that the ecological habitats of DNRA bacteria in anthropogenic ecosystems were far more abundant than previously assumed. However, the contribution to N transformation by the widespread DNRA was not significant in traditional municipal WWTPs.