Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


HAND


Experimental setup


Influent:Synthetic wastewater simulating municipal wastewater

HN-AD system:Heterotrophic nitrification/aerobic denitrification

Reactor:Sequencing batch reactor

Medium:Suspended-sludge

Culture taken from:Activated sludge sample from oil wastewater treatment station

Microorganism cultured:Pseudomonas stutzeri UFV5

Respiration:Aerobic

Electron donor:Pyruvate, acetate, citrate or sodium succinate

Electron acceptor:Ammonium

PH:7–9

Temperature:20–40?°C

HRT:72 h

NH4–N Influent conc(mg/L):1.32

NO2–N Influent conc(mg/L):nan

NO3–N Influent conc(mg/L):nan


Experimental Information


NH4–N Effluent (mg N/L):30.04?

NO2–N Effluent (mg N/L):nan

NO3-N Effluent (mg N/L):nan

NH4–N removal rate mg/L/d:nan

NO2–N removal rate mg/L/d:nan

NO3-N removal rate mg/L/d:nan

TN Removal rate (mg N/L/d):nan


Information about Article


Authors:Carneiro Fidélis Silva et al., 2020

Title:Physicochemical characterization of Pseudomonas stutzeri UFV5 and analysis of its transcriptome under heterotrophic nitrification/aerobic denitrification pathway induction condition

Pubmed link:Link

Full research link:Link

Abstract:Biological ammonium removal via heterotrophic nitrification/aerobic denitrification (HN/AD) presents several advantages in relation to conventional removal processes, but little is known about the microorganisms and metabolic pathways involved in this process. In this study, Pseudomonas stutzeri UFV5 was isolated from an activated sludge sample from oil wastewater treatment station and its ammonium removal via HN/AD was investigated by physicochemical and molecular approaches to better understand this process and optimize the biological ammonium removal in wastewater treatment plants. Results showed that P. stutzeri UFV5 removed all the ammonium in 48–72?hours using pyruvate, acetate, citrate or sodium succinate as carbon sources, C/N ratios 6, 8, 10 and 12, 3–6% salinities, pH 7–9 and temperatures of 20–40?°C. Comparative genomics and PCR revealed that genes encoding the enzymes involved in anaerobic denitrification process are present in P. stutzeri genome, but no gene that encodes enzymes involved in autotrophic nitrification was found. Furthermore, transcriptomics showed that none of the known enzymes of autotrophic nitrification and anaerobic denitrification had their expression differentiated and an upregulation of the biosynthesis machinery and protein translation was observed, besides several genes with unknown function, indicating a non-conventional mechanism involved in HN/AD process.