Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Raw secondary sewage treatment
HN-AD system:Heterotrophic nitrification/aerobic denitrification
Reactor:Sequencing batch reactor
Medium:Suspended-sludge
Culture taken from:Sediment of the eutrophic Taihu Lake
Microorganism cultured:Pseudomonas stutzeri YG-24
Respiration:Aerobic
Electron donor:Sodium citrate
Electron acceptor:Ammonium, Nitrate, Nitrite
PH:7.2
Temperature:28 °C
HRT:nan
NH4–N Influent conc(mg/L):nan
NO2–N Influent conc(mg/L):nan
NO3–N Influent conc(mg/L):nan
NH4–N Effluent (mg N/L):nan
NO2–N Effluent (mg N/L):nan
NO3-N Effluent (mg N/L):nan
NH4–N removal rate mg/L/d:8.75
NO2–N removal rate mg/L/d:7.51
NO3-N removal rate mg/L/d:7.73
TN Removal rate (mg N/L/d):0.8813
Authors:Li et al., 2015
Title:Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24
Pubmed link:None
Full research link:Link
Abstract:Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2?-N and NO3?-N removal rates of 8.75, 7.51 and 7.73 mg L?1 h?1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2?-N, NO3?-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2?-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.