Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Raw secondary sewage treatment

HN-AD system:Heterotrophic nitrification/aerobic denitrification

Reactor:Sequencing batch reactor


Culture taken from:Sediment of the eutrophic Taihu Lake

Microorganism cultured:Pseudomonas stutzeri YG-24


Electron donor:Sodium citrate

Electron acceptor:Ammonium, Nitrate, Nitrite


Temperature:28 °C


NH4–N Influent conc(mg/L):nan

NO2–N Influent conc(mg/L):nan

NO3–N Influent conc(mg/L):nan

Experimental Information

NH4–N Effluent (mg N/L):nan

NO2–N Effluent (mg N/L):nan

NO3-N Effluent (mg N/L):nan

NH4–N removal rate mg/L/d:8.75

NO2–N removal rate mg/L/d:7.51

NO3-N removal rate mg/L/d:7.73 

TN Removal rate (mg N/L/d):0.8813

Information about Article

Authors:Li et al., 2015

Title:Removal of nitrogen by heterotrophic nitrification–aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24

Pubmed link:None

Full research link:Link

Abstract:Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4+-N, NO2?-N and NO3?-N removal rates of 8.75, 7.51 and 7.73 mg L?1 h?1 were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4+-N, NO2?-N, NO3?-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4+-N to NO2?-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.