Influent:Synthetic wastewater simulating municipal wastewater
HN-AD system:Heterotrophic nitrification/aerobic denitrification
Reactor:Lab scale shaking flask batch
Medium:Suspended-sludge
Culture taken from:Pure culture
Microorganism cultured:Bacillus licheniformis
Respiration:Aerobic
Electron donor:nan
Electron acceptor:Ammonium
PH:7
Temperature:37°C
HRT:nan
NH4–N Influent conc(mg/L):30.0
NO2–N Influent conc(mg/L):30.0
NO3–N Influent conc(mg/L):30.0
NH4–N Effluent (mg N/L):nan
NO2–N Effluent (mg N/L):nan
NO3-N Effluent (mg N/L):nan
NH4–N removal rate mg/L/d:0.84
NO2–N removal rate mg/L/d:0.62
NO3-N removal rate mg/L/d:0.62
TN Removal rate (mg N/L/d):73%
Authors:Khanichaidecha et al., 2018
Title:Heterotrophic nitrification and aerobic denitrification using pure-culture bacteria for wastewater treatment
Pubmed link:None
Full research link:Link
Abstract:Due to the high water demand and unsustainable water resource, wastewater reclamation and wastewater treatment prior to discharge have become current important issues. Various treatment technologies, such as biological processes, have been improved as alternatives. In this study, the biological nitrogen removal system using pure-culture Bacillus licheniformis was developed and used as an internal treatment unit in an aquarium to improve the effluent quality for water reuse. The efficiencies for NH4-N and total nitrogen (TN) removal and the quality of treated water verified the occurrence of heterotrophic nitrification and aerobic denitrification; the nitrification rate was 0.84 mg/L-h and the denitrification rate was 0.62 mg/L-h. The maximal NH4-N and TN removal efficiencies were approximately 73% at the influent NH4-N of 30 mg/L. However, the other competitive heterotroph of Pseudomonas sp. was observed, which resulted in dramatically decreasing efficiencies and an enlarged ratio of carbon consumption and nitrogen removal. Although the overall performance of the B. licheniformis system was lower than the system using mixed-culture nitrifying and heterotrophic denitrifying microorganisms, the advantages of the B. licheniformis system were ease of operation and the fact that it is a land-limited treatment system. The research is ongoing to enhance performance and maintain excellent efficiency in a long-term operation.