Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Swine wastewater ponds

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:Bioreactor


Culture taken from:Swine wastewater ponds

Microorganism cultured:Rhodococcus sp. CPZ24


Electron donor:Ammonium sulphate

Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate


Experimental Information

Ammonia removal rate:3.1 mg L-1 h-1

Nitrate removal rate:0.93 mg L-1 h-1

Ammonia Removal efficiency (%):0.86

Nitrate Removal efficiency (%):0.67

Total Nitrogen Removal (%):0.48

Carbon Source:L-asparagine

Final products:Nitrogen, Nitrous oxide, Nitrite, Nitric oxide

Information about Article

Major findings:Rhodococcus sp. CPZ24 was a newly isolated bacterium from swine wastewater. It can remove nitrate and ammonium at a moderately high rate

Authors:Chen et al., 2012

Title:Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24

Pubmed link:Link

Full research link:Link

Abstract:Rhodococcus sp. CPZ24 was isolated from swine wastewater and identified. Batch (0.25 L flask) experiments of nitrogen removal under aerobic growth conditions showed complete removal of 50 mg L(-1) ammonium nitrogen within 20 h, while nitrate nitrogen removal reached 67%. A bioreactor (50 L) was used to further assess the heterotrophic nitrification and aerobic denitrification abilities of Rhodococcus sp. CPZ24. The results showed that 85% of the ammonium nitrogen (100 mg L(-1)) was transformed to nitrification products (NO(3)(-)-N and NO(2)(-)-N) (13%), intracellular nitrogen (24%), and gaseous denitrification products (48%) within 25 h. The ammonium nitrogen removal rate was 3.4 mg L(-1)h(-1). The results indicate that the strain CPZ24 carries out simultaneous nitrification and denitrification, demonstrating a potential use of the strain for wastewater treatment.