Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:Rotary shaker
Medium:Suspension
Culture taken from:nan
Microorganism cultured:Agrobacterium sp. LAD9
Respiration:Aerobic
Electron donor:Ammonium chloride
Electron acceptor:Oxygen, Sodium nitrite, Sodium nitrate
PH:8–10
Ammonia removal rate: n/a
Nitrate removal rate: n/a
Ammonia Removal efficiency (%): n/a
Nitrate Removal efficiency (%): n/a
Total Nitrogen Removal (%):0.501
Carbon Source:Sodium succinate
Final products:Nitrogen, Nitrous oxide
Major findings:The pathway of nitrogen removal in strain LAD9 was proposed: part of nitrogen was assimilated into the intracellular material; part of nitrogen was dissimilatorily reduced to gas products through heterotrophic nitrification coupled with aerobic denitrification, in which also accompanied with the mutual transformation of nitrite and nitrate. The findings can help in applying appropriate controls over operational parameters in systems involving the use of this kind of strain.
Authors:Chen and Ni. 2012
Title:Ammonium removal by Agrobacterium sp. LAD9 capable of heterotrophic nitrification-aerobic denitrification
Pubmed link:Link
Full research link:Link
Abstract:Characteristics of ammonium removal by a newly isolated heterotrophic nitrification-aerobic denitrification bacterium Agrobacterium sp. LAD9 were systematically investigated. Succinate and acetate were found to be the most favorable carbon sources for LAD9. Response surface methodology (RSM) analysis demonstrated that maximum removal of ammonium occurred under the conditions with an initial pH of 8.46, C/N ratio of 8.28, temperature of 27.9°C and shaking speed of 150rpm, where temperature and shaking speed produced the largest effect. Further nitrogen balance analysis revealed that 50.1% of nitrogen was removed as gas products and 40.8% was converted to the biomass. Moreover, the occurrence of aerobic denitrification was evidenced by the utilization of nitrite and nitrate as nitrogen sources, and the successful amplifications of membrane bound nitrate reductase and cytochrome cd(1) nitrite reductase genes from strain LAD9. Thus, the nitrogen removal in strain LAD9 was speculated to comply with the mechanism of heterotrophic nitrification coupled with aerobic denitrification (NH(4)(+)-NH(2)OH-NO(2)(-)-N(2)O-N(2)), in which also accompanied with the mutual transformation of nitrite and nitrate. The findings can help in applying appropriate controls over operational parameters in systems involving the use of this kind of strain.