Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Synthetic wastewater
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:Rotary shaker
Medium:Suspension
Culture taken from:Wastewater
Microorganism cultured:Bacillus methylotrophicus strain L7
Respiration:Aerobic
Electron donor:Ammonium sulphate
Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate
PH:7–8
Ammonia removal rate:51.58 mg L-1 h-1
Nitrate removal rate: 5.81 mg L-1 h-1
Ammonia Removal efficiency (%):0.834
Nitrate Removal efficiency (%):nan
Total Nitrogen Removal (%):nan
Carbon Source:Sodium succinate
Final products:Nitrogen, Nitrous oxide
Major findings:In this study, Bacillus methylotrophicus L7 was the first reported Gram-positive bacterial strain to denitrify nitrite to molecular nitrogen and denitrifying nitrite and nitrate to nitrous oxide in aerobic condition.
Authors:Zhang et al., 2012
Title:The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7
Pubmed link:Link
Full research link:Link
Abstract:Bacillus methylotrophicus strain L7, exhibited efficient heterotrophic nitrification-aerobic denitrification ability, with maximum NH(4)(+)-N and NO(2)(-)-N removal rate of 51.58 mg/L/d and 5.81 mg/L/d, respectively. Strain L7 showed different gaseous emitting patterns from those strains ever described. When (15)NH(4)Cl, or Na(15)NO(2), or K(15)NO(3) was used, results of GC-MS indicated that N(2)O was emitted as the intermediate of heterotrophic nitrification or aerobic denitrification, while GC-IRMS results showed that N(2) was produced as end product when nitrite was used. Single factor experiments suggested that the optimal conditions for heterotrophic nitrification were sodium succinate as carbon source, C/N 6, pH 7-8, 0 g/L NaCl, 37 °C and a wide range of NH(4)(+)-N from 80 to 1000 mg/L. Orthogonal tests showed that the optimal conditions for aerobic denitrification were C/N 20, pH 7-8, 10 g/L NaCl and DO 4.82 mg/L (shaking speed 50 r/min) when nitrite was served as substrate.