Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Recirculating water in a marine aquaculture system
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:Biological aerated filter
Medium:Suspension
Culture taken from:Biological aerated filter
Microorganism cultured:Marinobacter sp F6
Respiration:Aerobic
Electron donor:Ammonium sulphate
Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate
PH:7.5
Ammonia removal rate:nan
Nitrate removal rate:nan
Ammonia Removal efficiency (%):1
Nitrate Removal efficiency (%):1
Total Nitrogen Removal (%):0.4862
Carbon Source:Sodium succinate
Final products:Nitrogen, Nitrous oxide
Major findings:Strain F6 showed quite efficient ammonia and nitrate/nitrite removal under aerobic culture conditions. Strains like F6 likely play important roles in the nitrogen cycle of marine environments and may be applied in the nitrogen removal of high-salinity wastewater and marine culture wastewater.
Authors:Zheng et al., 2012
Title:Characterization of a marine origin aerobic nitrifying-denitrifying bacterium
Pubmed link:Link
Full research link:Link
Abstract:The bacterial strain F6 was isolated from a biological aerated filter that is used for purifying recirculating water in a marine aquaculture system and was identified as Marinobacter sp. based on the analysis of its 16S rRNA gene sequence. Strain F6 showed efficient aerobic denitrifying ability. One hundred percent of nitrates and 73.10% of nitrites were removed, and the total nitrogen (TN) removal rates reached 50.08% and 33.03% under a high nitrate and nitrite concentration in the medium, respectively. N(2)O and (15)N(2), as revealed by GC-MS and GC-IRMS, were the products of aerobic denitrification. Factors affecting the growth and aerobic denitrifying performance of strain F6 were investigated. The results showed that the optimum aerobic denitrification conditions for strain F6 were the presence of sodium succinate as a carbon source, a C/N ratio of 15, salinity ranging from 32-35 g/L of NaCl, incubation temperature of 30°C, an initial pH of 7.5, and rotation speed of 150 rpm [dissolved oxygen (DO) 6.75 mg/L]. In addition, strain F6 was confirmed to be a heterotrophic nitrifier through its NO(2)(-) generation and 25.96% TN removal when NH(4)(+) was used as the sole N source. Therefore, strain F6, the first reported member of genus Marinobacter with aerobic heterotrophic nitrifying-denitrifying ability, is an excellent candidate for facilitating simultaneous nitrification and denitrification (SND) in industry and aquaculture wastewater.