Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Saltern pool
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:nan
Medium:Suspension
Culture taken from:Sediment
Microorganism cultured:Halomonas sp. strain B01
Respiration:Aerobic
Electron donor:Ammonium sulphate
Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate
PH:7
Ammonia removal rate:nan
Nitrate removal rate:nan
Ammonia Removal efficiency (%):0.755
Nitrate Removal efficiency (%):0.727
Total Nitrogen Removal (%):0.96
Carbon Source:Glucose
Final products:Nitrogen
Major findings:The strains identified in this study have unique potential in SND removal in high salt and ammonium wastewater.
Authors:Wang et al., 2017
Title:Simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of NaCl and ammonia nitrogen by Halomonas bacteria
Pubmed link:Link
Full research link:Link
Abstract:To improve the efficiency of simultaneous heterotrophic nitrification and aerobic denitrification (SND) at high concentrations of NaCl and ammonia nitrogen (NH4+-N), we investigated the SND characteristics of Halomonas bacteria with the ability to synthesize the compatible solute ectoine. Halomonas sp. strain B01, which was isolated, screened and identified in this study, could simultaneously remove nitrogen (N) by SND and synthesize ectoine under high NaCl conditions. Gene cloning and sequencing analysis indicated that this bacterial genome contains ammonia monooxygenase (amoA) and nitrate reductase (narH) genes. Optimal conditions for N removal in a solution containing 600 mg/L NH4+-N were as follows: sodium succinate supplied as organic carbon (C) source at a C/N ratio of 5, pH 8 and shaking culture at 90 rpm. The N removal rate was 96.0% under these conditions. The SND by Halomonas sp. strain B01 was performed in N removal medium containing 60 g/L NaCl and 4,000 mg/L NH4+-N; after 180 h the residual total inorganic N concentration was 21.7 mg/L and the N removal rate was 99.2%. Halomonas sp. strain B01, with the ability to synthesize the compatible solute ectoine, could simultaneously tolerate high concentrations of NaCl and NH4+-N and efficiently perform N removal by SND.