Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Saline wastewater

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:Aerated biological filter reactors

Medium:Immobilized polyvinyl alcohol (PVA) beads

Culture taken from:Marine sediment

Microorganism cultured:Vibrio diabolicus SF16


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate


Experimental Information

Ammonia removal rate:nan

Nitrate removal rate:nan

Ammonia Removal efficiency (%):0.9182

Nitrate Removal efficiency (%):0.9971

Total Nitrogen Removal (%):0.7392

Carbon Source:Sodium acetate

Final products:Nitrogen

Information about Article

Major findings:The isolated strain showed excellent heterotrophic nitrifying–aerobic denitrifying ability, with nitrogen gas as the main of gaseous denitrification product. An aerated biological filter system inoculated with strain SF16 was employed to treat saline wastewater and showed potential promising for industrial and agricultural applications.

Authors:Duan et al., 2015

Title:Characterization of a halophilic heterotrophic nitrification–aerobic denitrification bacterium and its application on treatment of saline wastewater

Pubmed link:Link

Full research link:Link

Abstract:A novel halophilic bacterium capable of heterotrophic nitrification–aerobic denitrification was isolated from marine sediments and identified as Vibrio diabolicus SF16. It had ability to remove 91.82% of NH4+-N (119.77 mg/L) and 99.71% of NO3?-N (136.43 mg/L). The nitrogen balance showed that 35.83% of initial NH4+-N (119.77 mg/L) was changed to intracellular nitrogen, and 53.98% of the initial NH4+-N was converted to gaseous denitrification products. The existence of napA gene further proved the aerobic denitrification ability of strain SF16. The optimum culture conditions were salinity 1–5%, sodium acetate as carbon source, C/N 10, and pH 7.5–9.5. When an aerated biological filter system inoculated with strain SF16 was employed to treat saline wastewater, the average removal efficiency of NH4+-N and TN reached 97.14% and 73.92%, respectively, indicating great potential of strain SF16 for future full-scale applications.