Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Synthetic wastewater

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:Rotary shaker


Culture taken from:Activated biomass from a common effluent treatment plant (CETP) treating wastes from chemical and dye industrie

Microorganism cultured:Diaphorobacter sp.


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Sodium nitrite, Potassium nitrate


Experimental Information

Ammonia removal rate:nan

Nitrate removal rate:nan

Ammonia Removal efficiency (%):92–96%

Nitrate Removal efficiency (%):nan

Total Nitrogen Removal (%):nan

Carbon Source:Sodium citrate

Final products:Nitrite, Nitrogen

Information about Article

Major findings:Considering the rarity of this genus in effluent treatment plants, the revealed a relatively high number of isolates of genus Diaphorobacter with capability of carrying out SND at different rates. 

Authors:Khardenavis et al., 2007

Title:Simultaneous nitrification and denitrification by diverse Diaphorobacter sp

Pubmed link:Link

Full research link:Link

Abstract:Eight bacterial isolates closely related to Diaphorobacter sp. were isolated from activated biomass surviving on wastewater laden with dyes and nitro-substituted chemicals and were identified by 16S rDNA sequence analysis. The isolates showed sequence similarity of 99-100% to other Diaphorobacter strains such as ZY 2006b, F2, NA5, PCA039, D. nitroreducens KSP4, and KSP3 and 98-99% sequence homology to D. nitroreducens NA10B (type strain JCM 11421). Neighbor-joining tree revealed that all the eight strains formed tight cluster together and also showed close clustering with other Diaphorobacter strains. Isolates demonstrated the ability to perform simultaneous nitrification and denitrification under aerobic conditions. Strains HPC 805, 815, 821, and 856 gave highest chemical oxygen demand removal (85-93%) and ammonia removal (92-96%), which correlated well with higher growth rates of the cultures. Simultaneously, complete removal of nitrate supplied in the medium in presence of ammonium and acetate (electron donor) was observed in addition to aerobic nitrite release from ammonium. Thus, the above strains showed ability to perform partial nitrification followed by further aerobic removal of common intermediate nitrite, which indicated their potential application in treatment systems for treatment of high-nitrogen-containing wastewaters.