Influent:Sediment of the eutrophic Taihu Lake
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:nan
Medium:nan
Culture taken from:Sediment
Microorganism cultured:Pseudomonas stutzeri YG-24
Respiration:Aerobic
Electron donor:Ammonium chloride
Electron acceptor:Oxygen, Sodium nitrite, Sodium nitrate
PH:7.2–7.3
Ammonia removal rate:8.75 mg L-1 h-1
Nitrate removal rate:7.73 mg L-1 h-1
Ammonia Removal efficiency (%):0.8813
Nitrate Removal efficiency (%):0.7083
Total Nitrogen Removal (%):0.8528
Carbon Source:Sodium citrate, Sodium succinate, Sodium acetate, Glucose and Sucrose
Final products:Nitrogen, Nitrous oxide, Nitrite, Nitric oxide
Major findings:Phosphate accumulating bacterium P. stutzeri YG-24 presented great ability to remove nitrogen in synthetic and real wastewater. It eliminated NH+ 4 -N via a pathway of NH+ 4 -N to NO2-N and to gaseous nitrogen which was a valuable feature for reliable sewage treatment
Authors:Li et al., 2015
Title:Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24
Pubmed link:Link
Full research link:Link
Abstract:Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.