Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Sediment of the eutrophic Taihu Lake

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:nan


Culture taken from:Sediment

Microorganism cultured:Pseudomonas stutzeri YG-24


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Sodium nitrite, Sodium nitrate


Experimental Information

Ammonia removal rate:8.75 mg L-1 h-1

Nitrate removal rate:7.73 mg L-1 h-1

Ammonia Removal efficiency (%):0.8813

Nitrate Removal efficiency (%):0.7083

Total Nitrogen Removal (%):0.8528

Carbon Source:Sodium citrate, Sodium succinate, Sodium acetate, Glucose and Sucrose

Final products:Nitrogen, Nitrous oxide, Nitrite, Nitric oxide

Information about Article

Major findings:Phosphate accumulating bacterium P. stutzeri YG-24 presented great ability to remove nitrogen in synthetic and real wastewater. It eliminated NH+ 4 -N via a pathway of NH+ 4 -N to NO2-N and to gaseous nitrogen which was a valuable feature for reliable sewage treatment

Authors:Li et al., 2015

Title:Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24

Pubmed link:Link

Full research link:Link

Abstract:Phosphate accumulating bacterium Pseudomonas stutzeri YG-24 exhibited efficient heterotrophic nitrification and aerobic denitrification ability. Single factor experiments showed that both heterotrophic nitrification and aerobic denitrification occurred with sodium citrate as carbon source and lower C/N ratio of 8. High average NH4(+)-N, NO2(-)-N and NO3(-)-N removal rates of 8.75, 7.51 and 7.73 mg L(-1)h(-1) were achieved. The application of strain YG-24 in wastewater samples resulted in TN, NH4(+)-N, NO2(-)-N, NO3(-)-N and P removal efficiencies of 85.28%, 88.13%, 86.15%, 70.83% and 51.21%. Sequencing and quantitative amplification by real-time PCR of napA, nirS and ppk showed that nitrogen removal pathway of strain YG-24 was achieved through heterotrophic ammonium nitrification coupled with fast nitrite denitrification (NH4(+)-N to NO2(-)-N and then to gaseous nitrogen) directly. These results demonstrated the strain as a suitable candidate to simultaneously remove both nitrogen and phosphate in wastewater treatment.