Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Marine sponge H. perleve collected from coast intertidal

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:nan


Culture taken from:Hymeniacidon perleve

Microorganism cultured:Pseudomonas sp. ADN-42


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Sodium nitrate


Experimental Information

Ammonia removal rate:6.52 mg L-1 h-1

Nitrate removal rate:nan

Ammonia Removal efficiency (%):0.998

Nitrate Removal efficiency (%):nan

Total Nitrogen Removal (%):0.779

Carbon Source:Sodium citrate, Sodium acetate

Final products:Nitrogen, Carbon dioxide

Information about Article

Major findings:The strain ADN-42 isolated from a marine sponge could degrade ammonia nitrogen by SND in the presence of pure oxygen with the production of N2 and CO2. And no greenhouse gas N2O was determined during the whole operation. This study suggested that continuous low DO may be better for denitrification, it was better to keep DO at 3 mg/L.

Authors:Jin et al., 2015

Title:Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42

Pubmed link:Link

Full research link:Link

Abstract:Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42 was very high. And the highest removal rate was 6.52 mg/L · h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO >8 mg/L), NH3-N removal efficiency was 56.9 %. Moreover, 38.4 % of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.