Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Marine sponge H. perleve collected from coast intertidal
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:nan
Medium:nan
Culture taken from:Hymeniacidon perleve
Microorganism cultured:Pseudomonas sp. ADN-42
Respiration:Aerobic
Electron donor:Ammonium chloride
Electron acceptor:Oxygen, Sodium nitrate
PH:7.0–8.5
Ammonia removal rate:6.52 mg L-1 h-1
Nitrate removal rate:nan
Ammonia Removal efficiency (%):0.998
Nitrate Removal efficiency (%):nan
Total Nitrogen Removal (%):0.779
Carbon Source:Sodium citrate, Sodium acetate
Final products:Nitrogen, Carbon dioxide
Major findings:The strain ADN-42 isolated from a marine sponge could degrade ammonia nitrogen by SND in the presence of pure oxygen with the production of N2 and CO2. And no greenhouse gas N2O was determined during the whole operation. This study suggested that continuous low DO may be better for denitrification, it was better to keep DO at 3 mg/L.
Authors:Jin et al., 2015
Title:Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42
Pubmed link:Link
Full research link:Link
Abstract:Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42 was very high. And the highest removal rate was 6.52 mg/L · h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO >8 mg/L), NH3-N removal efficiency was 56.9 %. Moreover, 38.4 % of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.