Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Raw water

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:nan


Culture taken from:Raw water

Microorganism cultured:Acinetobacter sp. Y16


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Nitrite, Nitrate


Experimental Information

Ammonia removal rate:1.03 ± 0.15 mg L-1 h-1

Nitrate removal rate:nan

Ammonia Removal efficiency (%):66.00 ± 4.16%

Nitrate Removal efficiency (%):56.73 ± 4.19%

Total Nitrogen Removal (%):66.00 ± 4.16%

Carbon Source:Sodium acetate

Final products:Nitrogen

Information about Article

Major findings:A novel Acinetobacter sp. Y16 was isolated from oligotrophic niche. It exhibited efficient ammonium removal ability at 2C, with nitrogen gas as the end gaseous product in aerobic conditions. The nitrogen metabolic pathway for strain Y16 was heterotrophic nitrification coupled with aerobic nitrite/nitrate denitrification. The strong adaptability of strain Y16 to low C/N ratio and low temperature conditions made it a promising candidate for treating micro-polluted source water in cold regions

Authors:Huang et al., 2013

Title:Ammonium removal by a novel oligotrophic Acinetobacter sp. Y16 capable of heterotrophic nitrification-aerobic denitrification at low temperature

Pubmed link:Link

Full research link:Link

Abstract:Ammonium removal from source water is usually inhibited by insufficient carbon sources and low temperature in Northeastern China. A strain Y16 was isolated from oligotrophic niche and was identified as Acinetobacter sp. Y16. It demonstrated excellent capability for ammonium removal at 2 °C, and simultaneously produced nitrogen gas as the end product. About 66% of ammonium was removed after 36 h of incubation. Only trace accumulation of nitrate was observed during the process. The utilization of nitrite and nitrate as well as the existence of napA gene further proved the aerobic denitrification ability of strain Y16. Sodium acetate was the most favorable carbon source for ammonium oxidation by strain Y16. High rotation speed was beneficial for ammonium oxidation. Furthermore, strain Y16 could efficiently remove ammonium at low C/N ratio and low temperature conditions, which was advantageous for nitrogen removal from source water under cold temperatures.