Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Piggery wastewater treatment system
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:Laboratory-scale sequencing batch reactor (SBR)
Medium:Suspension
Culture taken from:Activated sludge
Microorganism cultured:Acinetobacter junii YB
Respiration:Aerobic
Electron donor:Ammonium sulphate
Electron acceptor:Oxygen, Potassium nitrate
PH:7.5–8.5
Ammonia removal rate:5.56 mg L-1 h-1
Nitrate removal rate:9.92 mg L-1 h-1
Ammonia Removal efficiency (%):0.9991
Nitrate Removal efficiency (%):0.9905
Total Nitrogen Removal (%):0.783
Carbon Source:Sodium succinate
Final products:Nitrogen, Hydroxylamine, Nitrite, Nitric oxide
Major findings:A. junii YB is a newly isolated bacterium from activated sludge, was found to exhibit efficient heterotrophic nitrification–aerobic denitrification ability at a wide range of ammonium loads. Meanwhile, hydroxylamine, nitrite and nitrate could be metabolized by strain YB. The putative nitrogen pathway of the isolate could be heterotrophic nitrification coupled with aerobic denitrification
Authors:Ren et al., 2014
Title:The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB
Pubmed link:Link
Full research link:Link
Abstract:A novel heterotrophic nitrifying bacterium was isolated from activated sludge and was identified as Acinetobacter junii YB. The strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability at a broad range of ammonium loads and had the capability to utilize hydroxylamine, nitrite and nitrate as a sole nitrogen source. Based on the nitrogen removal and enzyme assay, the nitrogen removal pathway was speculated to be achieved through heterotrophic nitrification coupled with aerobic denitrification. In addition, single-factor experiments showed that efficient heterotrophic nitrification and growth of strain YB occurred with succinate as the carbon source, pH 7.5, 37 °C, and high C/N ratio and dissolved oxygen. Furthermore, the new isolate showed capacities for aggregation and hydrophobicity. Regular variations of the flocculating ability and relative hydrophobicity were observed during the whole cultivation. The ability to perform heterotrophic nitrification-aerobic denitrification and cell aggregation demonstrated the great potential of the strain YB for future applications.