Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Piggery wastewater treatment system

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:Laboratory-scale sequencing batch reactor (SBR)


Culture taken from:Activated sludge

Microorganism cultured:Acinetobacter junii YB


Electron donor:Ammonium sulphate

Electron acceptor:Oxygen, Potassium nitrate


Experimental Information

Ammonia removal rate:5.56 mg L-1 h-1

Nitrate removal rate:9.92 mg L-1 h-1

Ammonia Removal efficiency (%):0.9991

Nitrate Removal efficiency (%):0.9905

Total Nitrogen Removal (%):0.783

Carbon Source:Sodium succinate

Final products:Nitrogen, Hydroxylamine, Nitrite, Nitric oxide

Information about Article

Major findings:A. junii YB is a newly isolated bacterium from activated sludge, was found to exhibit efficient heterotrophic nitrification–aerobic denitrification ability at a wide range of ammonium loads. Meanwhile, hydroxylamine, nitrite and nitrate could be metabolized by strain YB. The putative nitrogen pathway of the isolate could be heterotrophic nitrification coupled with aerobic denitrification

Authors:Ren et al., 2014

Title:The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium, Acinetobacter junii YB

Pubmed link:Link

Full research link:Link

Abstract:A novel heterotrophic nitrifying bacterium was isolated from activated sludge and was identified as Acinetobacter junii YB. The strain exhibited efficient heterotrophic nitrification-aerobic denitrification ability at a broad range of ammonium loads and had the capability to utilize hydroxylamine, nitrite and nitrate as a sole nitrogen source. Based on the nitrogen removal and enzyme assay, the nitrogen removal pathway was speculated to be achieved through heterotrophic nitrification coupled with aerobic denitrification. In addition, single-factor experiments showed that efficient heterotrophic nitrification and growth of strain YB occurred with succinate as the carbon source, pH 7.5, 37 °C, and high C/N ratio and dissolved oxygen. Furthermore, the new isolate showed capacities for aggregation and hydrophobicity. Regular variations of the flocculating ability and relative hydrophobicity were observed during the whole cultivation. The ability to perform heterotrophic nitrification-aerobic denitrification and cell aggregation demonstrated the great potential of the strain YB for future applications.