Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Experimental setup

Influent:Domestic sewage water

Anammox system:Simultaneous nitrification and denitrification (SND)

Anammox reactor:Laboratory-scale sequencing batch reactor (SBR)


Culture taken from:Enrichment culture

Microorganism cultured:Klebsiella pneumoniae CF-S9


Electron donor:Ammonium chloride

Electron acceptor:Oxygen, Sodium nitrite, Sodium nitrate


Experimental Information

Ammonia removal rate:4.3 mg L-1 h-1

Nitrate removal rate:2.2 mg L-1 h-1

Ammonia Removal efficiency (%):0.85

Nitrate Removal efficiency (%):nan

Total Nitrogen Removal (%):nan

Carbon Source:Acetamide

Final products:Nitrogen

Information about Article

Major findings:The present investigation demonstrated that ammonia, nitrite and nitrate could all be used by the isolate under aerobic condition and hydroxylamine reductase, nitrate reductase and nitrite reductase were expressed in cell periplasm. Expression of nir enzyme is suggestive of transformation of nitrite to either nitric oxide or nitrous oxide

Authors:Padhi et al., 2013

Title:Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater

Pubmed link:None

Full research link:Link

Abstract:Nitrogen pollution has been serious problem in environmental water particularly in freshwater ecosystems. A heterotrophic nitrifier and aerobic denitrifier was isolated and characterised from domestic wastewater. Based on phenotypic and phylogenetic characteristics, the isolate was identified as Klebsiella pneumonia. The strain had the capability to utilise ammonia, nitrate and nitrite as a sole nitrogen source. The maximum growth rate (?max) of isolate was 0.55 h?1 when ammonia used as nitrogen source. The isolate could express hydroxylamine oxidase (hao), periplasmic nitrate reductase (nap) and nitrite reductase (nir) which are essential for heterotrophic nitrification and aerobic denitrification. Both hao and napA genes were amplified in isolated strain by PCR. Furthermore, the isolate showed capacities of extracellular polymeric substances (EPS) secretion and auto-aggregation. Results demonstrated that the isolation of heterotrophic nitrifier and aerobic denitrifier favoured the bioremediation of nitrogenous compounds from domestic wastewater.