Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Biological Nitrogen Removal Database
A manually curated data resource for microbial nitrogen removal
Influent:Domestic sewage water
Anammox system:Simultaneous nitrification and denitrification (SND)
Anammox reactor:Laboratory-scale sequencing batch reactor (SBR)
Medium:Suspension
Culture taken from:Enrichment culture
Microorganism cultured:Klebsiella pneumoniae CF-S9
Respiration:Aerobic
Electron donor:Ammonium chloride
Electron acceptor:Oxygen, Sodium nitrite, Sodium nitrate
PH:7.1–7.2
Ammonia removal rate:4.3 mg L-1 h-1
Nitrate removal rate:2.2 mg L-1 h-1
Ammonia Removal efficiency (%):0.85
Nitrate Removal efficiency (%):nan
Total Nitrogen Removal (%):nan
Carbon Source:Acetamide
Final products:Nitrogen
Major findings:The present investigation demonstrated that ammonia, nitrite and nitrate could all be used by the isolate under aerobic condition and hydroxylamine reductase, nitrate reductase and nitrite reductase were expressed in cell periplasm. Expression of nir enzyme is suggestive of transformation of nitrite to either nitric oxide or nitrous oxide
Authors:Padhi et al., 2013
Title:Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater
Pubmed link:None
Full research link:Link
Abstract:Nitrogen pollution has been serious problem in environmental water particularly in freshwater ecosystems. A heterotrophic nitrifier and aerobic denitrifier was isolated and characterised from domestic wastewater. Based on phenotypic and phylogenetic characteristics, the isolate was identified as Klebsiella pneumonia. The strain had the capability to utilise ammonia, nitrate and nitrite as a sole nitrogen source. The maximum growth rate (?max) of isolate was 0.55 h?1 when ammonia used as nitrogen source. The isolate could express hydroxylamine oxidase (hao), periplasmic nitrate reductase (nap) and nitrite reductase (nir) which are essential for heterotrophic nitrification and aerobic denitrification. Both hao and napA genes were amplified in isolated strain by PCR. Furthermore, the isolate showed capacities of extracellular polymeric substances (EPS) secretion and auto-aggregation. Results demonstrated that the isolation of heterotrophic nitrifier and aerobic denitrifier favoured the bioremediation of nitrogenous compounds from domestic wastewater.